Cressiot, Benjamin, Greive, Sandra Joy Muriatu orcid.org/0000-0001-6067-2632, Mojtabavi, Mehrnaz et al. (2 more authors) (2018) Thermostable virus portal proteins as reprogrammable adapters for solid-state nanopore sensors. Nature Communications. 4652. pp. 1-7. ISSN 2041-1723
Abstract
Nanopore-based sensors are advancing the sensitivity and selectivity of single-molecule detection in molecular medicine and biotechnology. Current electrical sensing devices are based on either membrane protein pores supported in planar lipid bilayers or solid-state (SS) pores fabricated in thin metallic membranes. While both types of nanosensors have been used in a variety of applications, each has inherent disadvantages that limit its use. Hybrid nanopores, consisting of a protein pore supported within a SS membrane, combine the robust nature of SS membranes with the precise and simple engineering of protein nanopores. We demonstrate here a novel lipid-free hybrid nanopore comprising a natural DNA pore from a thermostable virus, electrokinetically inserted into a larger nanopore supported in a silicon nitride membrane. The hybrid pore is stable and easy to fabricate, and, most importantly, exhibits low peripheral leakage allowing sensing and discrimination among different types of biomolecules.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018, The Author(s). |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 12 Nov 2018 15:40 |
Last Modified: | 06 Dec 2023 12:49 |
Published Version: | https://doi.org/10.1038/s41467-018-07116-x |
Status: | Published |
Refereed: | Yes |
Identification Number: | https://doi.org/10.1038/s41467-018-07116-x |
Related URLs: |