Buswell, W., Schwarzenbacher, E.R., Luna, E. et al. (5 more authors) (2018) Chemical priming of immunity without costs to plant growth. New Phytologist, 218 (3). pp. 1205-1216. ISSN 0028-646X
Abstract
- β-aminobutyric acid (BABA) induces broad-spectrum disease resistance, but also represses plant growth, which has limited its exploitation in crop protection. BABA perception relies on binding to the aspartyl-tRNA synthetase (AspRS) IBI1, which primes the enzyme for secondary defense activity. This study aimed to identify structural BABA analogues that induce resistance without stunting plant growth.
- Using site-directed mutagenesis, we demonstrate that the (L)-aspartic acid-binding domain of IBI1 is critical for BABA perception. Based on interaction models of this domain, we screened a small library of structural BABA analogues for growth repression and induced resistance against biotrophic Hyaloperonospora arabidopsidis (Hpa).
- A range of resistance-inducing compounds were identified, of which (R)-β-homoserine (RBH) was the most effective. Surprisingly, RBH acted through different pathways than BABA. RBH-induced resistance (RBH-IR) against Hpa functioned independently salicylic acid, partially relied on camalexin, and was associated with augmented cell wall defense. RBH-IR against necrotrophic Plectosphaerella cucumerina acted via priming of ethylene and jasmonic acid defenses. RBH-IR was also effective in tomato against Botrytis cinerea. Metabolic profiling revealed that RBH, unlike BABA, does not majorly affect plant metabolism.
- RBH primes distinct defense pathways against biotrophic and necrotrophic pathogens without stunting plant growth, signifying strong potential for exploitation in crop protection.
Metadata
Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 The Authors. New Phytologist © 2018 New Phytologist Trust. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | ||||||||||
Keywords: | β-homoserine; β-aminobutyric acid; IBI1; induced resistance; priming; crop protection | ||||||||||
Dates: |
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Institution: | The University of Sheffield | ||||||||||
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Animal and Plant Sciences (Sheffield) | ||||||||||
Funding Information: |
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Depositing User: | Symplectic Sheffield | ||||||||||
Date Deposited: | 22 Jan 2018 11:41 | ||||||||||
Last Modified: | 10 Nov 2020 17:16 | ||||||||||
Status: | Published | ||||||||||
Publisher: | Wiley | ||||||||||
Refereed: | Yes | ||||||||||
Identification Number: | https://doi.org/10.1111/nph.15062 |