Rosenzweig, Elizabeth S Freeman, Xu, Bin, Cuellar, Luis Kuhn et al. (11 more authors) (2017) The eukaryotic CO2-concentrating organelle is liquid-like and exhibits dynamic reorganization. Cell. 148-162.e19. ISSN 1097-4172
Abstract
Approximately 30%–40% of global CO 2 fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO 2-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a “magic number” effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2017 Elsevier Inc. All rights reserved. |
Keywords: | CO concentrating mechanism, Chlamydomonas reinhardtii, Rubisco, biological phase transitions, carbon fixation, cryo-electron tomography, liquid-like organelles, magic numbers, organelle inheritance, pyrenoid, Chloroplasts/chemistry, Cryoelectron Microscopy, Organelle Biogenesis, Carbon Dioxide/metabolism, Ribulose-Bisphosphate Carboxylase/metabolism, Chlamydomonas reinhardtii/chemistry, Algal Proteins/metabolism |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 30 Aug 2017 08:00 |
Last Modified: | 25 Mar 2024 00:08 |
Published Version: | https://doi.org/10.1016/j.cell.2017.08.008 |
Status: | Published |
Refereed: | Yes |
Identification Number: | https://doi.org/10.1016/j.cell.2017.08.008 |
Related URLs: |
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