QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids

Jajesniak, P. and Wong, T.S. (2015) QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids. Journal of Biological Engineering, 9 (15). ISSN 1754-1611

Abstract

Metadata

Authors/Creators:
  • Jajesniak, P.
  • Wong, T.S.
Copyright, Publisher and Additional Information: © 2015 Jajesniak and Wong. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Keywords: Molecular cloning; Gene cloning; Megaprimer; Recombinant DNA; Recombinant plasmid; Protein engineering; Directed evolution; Synthetic biology; Metabolic engineering
Dates:
  • Published: 18 September 2015
  • Published (online): 18 September 2015
  • Accepted: 21 July 2015
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield)
Depositing User: Symplectic Sheffield
Date Deposited: 26 Jul 2017 13:59
Last Modified: 26 Jul 2017 13:59
Published Version: https://doi.org/10.1186/s13036-015-0010-3
Status: Published
Publisher: BioMed Central
Refereed: Yes
Identification Number: https://doi.org/10.1186/s13036-015-0010-3

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