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Prepatterning in the Stem Cell Compartment

Tonge, P.D., Olariu, V., Coca, D., Kadirkamanathan, V., Burrell, K., Billings, S.A. and Andrews, P.A. (2010) Prepatterning in the Stem Cell Compartment. PLoS ONE, 5 (e19090). ISSN 1932-6203

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The mechanism by which an apparently uniform population of cells can generate a heterogeneous population of differentiated derivatives is a fundamental aspect of pluripotent and multipotent stem cell behaviour. One possibility is that the environment and the differentiation cues to which the cells are exposed are not uniform. An alternative, but not mutually exclusive possibility is that the observed heterogeneity arises from the stem cells themselves through the existence of different interconvertible substates that pre-exist before the cells commit to differentiate. We have tested this hypothesis in the case of apparently homogeneous pluripotent human embryonal carcinoma (EC) stem cells, which do not follow a uniform pattern of differentiation when exposed to retinoic acid. Instead, they produce differentiated progeny that include both neuronal and non-neural phenotypes. Our results suggest that pluripotent NTERA2 stem cells oscillate between functionally distinct substates that are primed to select distinct lineages when differentiation is induced.

Item Type: Article
Copyright, Publisher and Additional Information: Copyright: © 2010 Tonge et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: cell population, differentiation
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Biomedical Science (Sheffield)
The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Automatic Control and Systems Engineering (Sheffield)
Funding Information:
FunderGrant number
Depositing User: Professor Peter Andrews
Date Deposited: 28 Jun 2010 17:11
Last Modified: 06 Jun 2014 09:07
Published Version: http://dx.doi.org/10.1371/journal.pone.0010901
Status: Published
Publisher: Public Library of Science
Refereed: Yes
Identification Number: 10.1371/journal.pone.0010901
URI: http://eprints.whiterose.ac.uk/id/eprint/10961

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