Hanotu, J., Kong, D. and Zimmerman, W.B. orcid.org/0000-0001-7123-737X (2016) Intensification of yeast production with microbubbles. Food and Bioproducts Processing, 100 (Part A). pp. 424-431. ISSN 0960-3085
Abstract
Yeast requires and consumes a high amount of oxygen rapidly during growth. Maintaining yeast cultures under sufficient aeration, however, is a significant challenge in yeast propagation. Due to their high surface area, microbubbles are more efficient in mass transfer than coarse bubbles. The performance of an airlift loop bioreactor equipped with a fluidic oscillator generated microbubbles in yeast propagation is presented here. The approach is compared with a conventional bubble generation method that produces coarse bubbles. Dosing with microbubbles transferred more oxygen to the cultures, achieving non-zero dissolved O2 levels and consequently, eliminating the starvation state of yeast in contrast to coarse bubble sparging. The average cell growth yield obtained under microbubble sparging reached 0.31 mg/h (±0.02) while 0.22 mg/h (±0.01) was recorded for cells grown with coarse bubbles during the log phase. The percent difference in average growth yield after 6 hours was 18%. Additionally, the use of microbubbles in yeast harvest from growth medium proved effective, yielding >99% cell recovery. The result of this study is crucial for the biofuel industry but also, the food, nutraceutical and pharmaceutical industry for which end product purity is premium.
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Copyright, Publisher and Additional Information: | © 2016 Elsevier. This is an author produced version of a paper subsequently published in Food and Bioproducts Processing. Uploaded in accordance with the publisher's self-archiving policy. Article available under the terms of the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/4.0/) | ||||||
Keywords: | Yeast Propagation; Fermentation; Microbubble; Airlift Loop Bioreactor (ALB); Microflotation | ||||||
Dates: |
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Institution: | The University of Sheffield | ||||||
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) | ||||||
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Depositing User: | Symplectic Sheffield | ||||||
Date Deposited: | 10 Oct 2016 12:27 | ||||||
Last Modified: | 31 Jul 2017 14:51 | ||||||
Published Version: | http://dx.doi.org/10.1016/j.fbp.2016.07.013 | ||||||
Status: | Published | ||||||
Publisher: | Elsevier | ||||||
Refereed: | Yes | ||||||
Identification Number: | https://doi.org/10.1016/j.fbp.2016.07.013 |