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Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in drosophila photoreceptors

Gonzalez-Bellido, P.T., Wardill, T.J., Kostyleva, R., Meinertzhagen, I.A. and Juusola, M. (2009) Overexpressing temperature-sensitive dynamin decelerates phototransduction and bundles microtubules in drosophila photoreceptors. Journal of Neuroscience, 29 (45). pp. 14199-14210. ISSN 0270-6474

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Abstract

shibire(ts1), a temperature-sensitive mutation of the Drosophila gene encoding a Dynamin orthologue, blocks vesicle endocytosis and thus synaptic transmission, at elevated, or restrictive temperatures. By targeted Gal4 expression, UAS-shibire(ts1) has been used to dissect neuronal circuits. We investigated the effects of UAS-shibire(ts1) overexpression in Drosophila photoreceptors at permissive (19 degrees C) and restrictive (31 degrees C) temperatures. At 19 degrees C, overexpression of UAS-shi(ts1) causes decelerated phototransduction and reduced neurotransmitter release. This phenotype is exacerbated with dark adaptation, age and in white mutants. Photoreceptors overexpressing UAS-shibire(ts1) contain terminals with widespread vacuolated mitochondria, reduced numbers of vesicles and bundled microtubules. Immuno-electron microscopy reveals that the latter are dynamin coated. Further, the microtubule phenotype is not restricted to photoreceptors, as UAS-shibire(ts1) overexpression in lamina cells also bundles microtubules. We conclude that dynamin has multiple functions that are interrupted by UAS-shibire(ts1) overexpression in Drosophila photoreceptors, destabilizing their neural communication irreversibly at previously reported permissive temperatures.

Item Type: Article
Copyright, Publisher and Additional Information: © 2009 Society for Neuroscience. Reproduced in accordance with the publisher's self-archiving policy.
Keywords: Long-term Adaption; Subcellular Translocation; Rhodopsin Endocytosis; TRPL Channels; Melanogaster; Shibire; Mutant; Histamine; Protein; Cell
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Science (Sheffield) > School of Biological Sciences (Sheffield) > Department of Biomedical Science (Sheffield)
Depositing User: Miss Anthea Tucker
Date Deposited: 02 Dec 2009 16:07
Last Modified: 07 Jun 2014 08:41
Published Version: http://dx.doi.org/10.1523/JNEUROSCI.2873-09.2009
Status: Published
Publisher: Society for Neuroscience
Refereed: Yes
Identification Number: 10.1523/JNEUROSCI.2873-09.2009
URI: http://eprints.whiterose.ac.uk/id/eprint/10223

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